The aim of the present study was to investigate whether dietary supplementa. Systolic blood pressure sbp and diastolic blood pressure dbp were then. Second, in one study in overweight men, the rise in ambulatory blood pressure in response to 200 g of oral fructose per day for two weeks was blocked in those subjects concomitantly administered allopurinol 26. Pdf dietary vitamin e and c supplementation prevents. A grape seed procyanidin extract ameliorates fructoseinduced. Chapter 8 effect of tender coconut water in high fructose. Male c57bl6 mice were weaned to a randomly assigned diet. In conclusion, taurine may ameliorate fructose induced hypertension in rats by preventing an increase in intracellular free calcium concentration. The addition of clonidine to the drinking water inhibited fructose induced hypertension, but not the increase in plasma insulin or triglyceride concentration seen in fructose fed rats. Prevention of fructoseinduced hypertension by dietary. Taken together, these data suggest that insulin resistance and diabetes are characterized. Fructose induces systemic hypertension through several mechanisms mainly associated.
Fructose induced hypertension has been shown to be attenuated in both glut5 and pat1 knockout mice 21,22. The magnitude of blood pressure of the c and ce did not alter during the period of experiment figure 1. Dec 11, 2014 indeed, several studies have pointed to the adverse cardiac and metabolic effects of a high fructose diet in animal models. Fructose induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats laura g. Male, wistar rats weighing 200250 g were used for this experiment. Cardiovascular disease cvd is the number one cause of premature mortality in the developed world, and hypertension is its most important risk factor. Fructoseinduced metabolic syndrome is associated with. The rats were divided into 4 groups of six each and fed the following diet. Fructose induces systemic hypertension through several mechanisms mainly associated with deleterious effects on target organs kidney, endothelium, heart.
Effect of aqueous extract of defatted flaxseeds linum. Fructose feeding can also cause insulin resistance and hyperinsulinemia in normal r a t s. Fructoseinduced hypertension has been shown to be attenuated in both glut5 and pat1 knockout mice 21,22. Characterized by increased insulin resistance, hyperinsulinemia, hypertension and hypertriglyceridemia. Rats were fed either a starch control diet or a diet containing 65% fructose for 8 weeks to induce hypertriglyceridemia.
Fructosefed rats and mice show moderate hypertension and glucose intolerance, associated with increased levels of plasma insulin, cholesterol and triglycerides 35. Feeding a highfructose diet induces hypertension and insulinresistance in. Read chronic n acetylcysteine prevents fructoseinduced insulin resistance and hypertension in rats, european journal of pharmacology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. In fructoseinduced hypertension in wistarkyoto wky rats, excess. In the present study, we examined the relationship between elevated insulin levels and hypertension in the fructose hypertensive rat model, in which the rise in blood pressure is induced by feeding normal rats a fructose enriched diet. Sixweekold spraguedawley rats were divided into four experimental groups. The relation between fructoseinduced metabolic syndrome and. Insulin resistance, hyperinsulinemia, hypertriglyceridemia, and mild hypertension have been induced in normotensive sprague dawley rats by simple sucrose or fructose feeding. Pdf fructoseinduced hypertension in rats is concentration. Effects of cyp2j3 gene delivery on cyp2j3 protein expression, urinary 14,15dhet levels, and fructose induced hypertension in rats. Serum uric acid remained unchanged in animals on increased fructose intake with hypertension. Hypertension is produced by the chronic administration of cdcl 1 mgkgday, i. If fructose stimulates salt absorption in the intestine and kidney, then it is reasonable to ask whether varying the amount of dietary salt has any impact on fructose induced hypertension. Effects of antihypertensive drugs on blood pressure and.
Fructoseinduced hypertension in rats is concentration. In contrast, mangiferin attenuated fructose induced acceleration of plasma nefa clearance during ogtt, and tended to decrease excessive triglyceride accumulation in gastrocnemius. In parallel studies, a chloridefree diet blocked fructoseinduced hypertension in sprague dawley rats. Fructose drinking rats had increased blood pressure and impaired acetylcholine induced relaxation of the thoracic aorta in comparison with control rats drinking just tap water. Materials and methods general protocol male spraguedawley rats simonsen laboratories, gilroy, ca, usa, initially weighing 160 to 180 g. Methanol extract of allanblackia gabonensis guttiferacae prevents hypertension and oxidative stress induced by chronic sucrose consumption in rat bilanda danielle claudea, andomo mewo yannic desirea, dzeu. The role of salt in the pathogenesis of fructoseinduced. Notably, switching to ls diet halted the pro gression of hf induced renal injuries. Hypertension was induced by administration of 10% wv solution of fructose orally in drinking water. To determine if hypertension could be produced in normal rats by feeding them a fructoseenriched diet, spraguedawley rats were fed either normal chow or a.
Whether dietary fructose as sucrose or high fructose corn syrup has unique effects separate from its role as carbohydrate, or, in fact, whether it can be considered inherently harmful, even a toxin, has assumed prominence in nutrition. A representative traces show the baseline renal sna in fructose fed rats throughout the 7 days of experiment. Fructose induced hypertension model gives clue about the role of dietary changes in hypertension. Fructoseinduced hypertension in rats is concentration and durationdependent soter dai and john h. Fructose feeding decreased salt excretion by the kidney and resulted in hypertension, a response almost abolished in the knockout mice.
Dietary lipoic acid supplementation prevents fructoseinduced. College of pharmacy, navarangpura, ahmedabad 380 009, india received 8 september 2004. Effects of the methanol extracts phaleria macrocarpascheff. Beneficial effects of zingiber officinale roscoe on fructose. In this study, the effects of coq10 on superoxide generation, downstream no signaling in the nts, and blood pressure were evaluated in rats with fructose.
We investigated whether fructose induced leptin resistance in mice could be used to study the metabolic consequences of fructose consumption in humans, particularly in children and adolescents. Jan 27, 2014 we examined in this study whether fructose affects atp content in rvlm and its significance in the increase in central sympathetic outflow and hypertension induced by the high fructose diet hfd. Asghar1, alysha thompson1, maggie chi1, andrew cusumano1, suzanne scheaffer1, noor alhammadi2, jessica l. Cinnamaldehyde and allopurinol reduce fructose induced cardiac hypertrophy and fibrosis in rats. Reaven summary to determine if hypertension could be produced in normal rats by feeding them a. Insulin resistance, hyperinsulinemia, hypertriglyceridemia and high blood pressure can be induced in fructosefed rats 46. Fructose also raises uric acid in the blood, she says, and that could raise blood pressure. The effect of various interventions on fructoseinduced hypertension and insulin resistance in rat. Regarding liver antioxidant status, oxidative stress was observed in fructose fed rats fgroup, while. Amplified vasopressor responses to minimal stimuli and differing responses to fructose in peripheral versus central sites may explain the controversy. In vivo antihypertensive and antihyperlipidemic effects of.
The objective of this study was to determine whether a grape seed procyanidin extract gspe exerts a triglyceridelowering effect in a hyperlipidemic state using the fructose fed rat model and to elucidate the underlying molecular mechanisms. The aim of this study was to evaluate antihypertensive and antihyperlipidemic effect in fructose induced hypertensive rats. The effect of hydroalcoholic celery apiumgraveolens. Male wistar rats were fed a high fructose 65 % diet or standard chow for 1 week, and treated with an ethyl acetate etoac extract of amla, a polyphenolrich fraction, at 10 or 20 mgkg body weight per d, or vehicle, for 2 weeks. Three months of highfructose feeding fails to induce. Cyp2j3 gene delivery reduces insulin resistance via. Oct 19, 2010 the association between fructose and increased blood pressure is still incompletely defined, because experimental studies have produced dissimilar conclusions.
Fructose can tell the kidneys to hold onto more salt, and that can contribute to high blood pressure. Antihypertensive effects of metformin in fructosefed. Dietary fructose and hypertension amplified vasopressor responses to minimal stimuli and differing responses to fructose in peripheral versus central sites may explain the controversy. Masoprocol lowers blood pressure in rats with fructoseinduced. Furthermore, clonidine blocks the hypertension, but not the metabolic effects of fructose. Rats were randomly divided into control and treatment groups n 6. Previous studies performed in animals have demonstrated the antihypertensive efficacy of etreceptor antagonists in doca salt rats, in dahlss rats, in hypertension induced by angiotensin ii infusion, and in rats with a high fructose diet, whereas these drugs were unable to lower blood pressure in shr and in renovascular models of. Metabolic syndrome and hypertension resulting from. Dec 21, 2011 effects of cyp2j3 gene delivery on cyp2j3 protein expression, plasma and urinary 14,15dhet levels. In wistar rats, 21 weeks of fructose supplementation induced a metabolic syndrome hypertension, insulin resistance, and dyslipidemia but not t2d. The most salient feature of the present studies is the prevention of generation of fructose induced hypertension in mice lacking slc26a6 and in rats on a chloridefree diet figures 4 and 5, clearly establishing the role of dietary chloride and chlorideabsorbing transporter slc26a6 in the pathogenesis of fructose induced hypertension.
An increase in adenosine5triphosphate atp content in. Cardiovascular effects of highfructose intake in rats. In conclusion, a 10% fructose solution in drinking water, which is equivalent to a diet containing 4857% calories value fructose, is superior to a 5% or 20% solution in the production of fructoseinduced hypertension in wistar rats that is associated with hyperinsulinemia, hyperglycemia, and hypertriglyceridemia. Fructoseinduced hypertension in rats is concentration and durationdependent. The animals were bred at the animal house of alameen college of pharmacy and reared as per the standard laboratory conditions. Group 1 control rats group 2 high fructose fed hypertensive rats. Sep 11, 2014 high fructose diets have been implicated in obesity via impairment of leptin signaling in humans and rodents. Ursodeoxycholic acid ameliorates fructoseinduced metabolic. Fructoseinduced hypertension has been shown to be attenuated in both glut5 and pat1 knockout mice. Pdf fructoseinduced hypertension in rats is concentration and. Our previous data indicated that fructose intake induced increased blood pressure, insulin resistance and downregulation of enos expression, and enos overexpression significantly decreased fructose induced hypertension and insulin resistance in rats. Therefore, the model of fructosedrinking rats is being widely used to induce the ms.
Sef was given orally once daily at the doses of 50,100 and 200mgkg. Studies have shown that high dosage of fructose induces hyperinsulinemia, hypertriglyceridemia, impaired glucose tolerance and hypertension in rats 5. Mitigation of insulin resistance by mangiferin in a rat. Mechanistically, mangiferin neither affected intakes of fructose and chow, and the increase in epididymal and perirenal fat, nor attenuated fructose induced hypertension. It has been demonstrated that cd36 expression is increased in the sarcolemma but not in whole skeletal muscle in fructose fed rats, suggesting that a fructose induced redistribution of this protein is associated with fatty acid uptake across the plasma membrane huynh et al. We investigated the effects of amla emblica officinalis gaertn. Beneficial effects of zingiber officinale roscoe on. Studies in rats have confirmed this speculation by showing that high salt intake enhances and low salt intake blunts the magnitude of fructose induced. Diabetes was induced into rats by the administration of fructose 10% in drinking water for six weeks. Systolic blood pressure in rats model of hypertension induced by fructose. In normotensive rats fed with high fructose diet hfd for 12 weeks, there was a significant increase in tissue atp content in rvlm, accompanied by. Pdf the present study determined the most suitable concentration and duration of fructose treatment for inducing hypertension in wistar rats.
Methanol extract of allanblackia gabonensis guttiferacae. Maternal fructose drives placental uric acid production leading to adverse fetal outcomes zeenat a. Pdf fructoseinduced insulin resistance and hypertension. Excessive fructose intake induces the features of metabolic. Increased cyp2j3 expression reduces insulin resistance in. Dietary lipoic acid supplementation prevents fructose. Thus, the metabolic changes associated with fructose induced hypertension are unlikely to be secondary to an increase in sympathetic activity. Increased fructose associates with elevated blood pressure. Rats fed a high fructose diet represent an animal model for insulin resistance and hypertension.
Preparation and antidiabetic effect of orally administered. Tr induced a reduction of fibroblast growth factor. Sucrose or fructose induced hypertension evidence for sucrose or fructose induced hypertension in animal models. Chapter 8 effect of tender coconut water in high fructose fed. In addition, hyperinsulinemia and hypertriglyceridemia were associated with hypertension in fructosefed rats. First, fructose induced hypertension in rats is largely ameliorated by lowering uric acid levels. Jul 01, 2010 july 1, 2010 foods and beverages with high amounts of fructose from added sugar may increase your risk of developing high blood pressure, according to a new study in the journal of the american. Fructose induced insulin resistance and hypertension in rats ishun hwang, helen ho, brian b. Metabolic syndrome and hypertension resulting from fructose. However, the molecular mechanisms by which coq10 modulates cardiovascular functions in the nts are unclear. Inhibitors of soluble epoxide hydrolase minimize ischemia. It is also well established that high fructose diets can induce hypertension in experimental animals, and in fact, fructose.
Mangiferin diminishes chronic fructose overconsumption induced cardiac fibrosis in spontaneous hypertension rats ling yao 1, chunli li2, ting chen1, weiguo cao, johji yamahara3, yuhao li1,4, yunmei zhang5, jianwei wang1 1chongqing key laboratory of traditional chinese medicine for prevention and cure of metabolic diseases. Further studies will be required to assess the contribution of insulin receptor abnormalities to the development of the hypertensive response to chronic fructose feeding in rats. A highfructose diet induces insulin resistance but not. Dietary fructose and hypertension, current hypertension. Mcneill division of pharmacology and toxicology, faculty of pharmaceutical sciences, the university of british columbia, vancouver, british columbia, canada the present study determined the most suitable concentration and duration of fructose treatment for inducing hypertension in wistar rats. In conclusion, this study indicates a possible cellular mechanism for the reduced sensitivity to insulin present in rats with fructose induced hypertension. Oct 30, 2009 fructose reduces the production of nitric oxide and makes it difficult for the vessels to relax and dilate. The addition of clonidine to the drinking water inhibited fructoseinduced hypertension, but not the increase in plasma insulin or triglyceride concentration seen in fructosefed rats. Gut dysbiosis contribute to high fructose induced salt. The average plasma fructose concentration in the fructose fed mice was 1550.
Therapeutic effects of tender coconut water on oxidative. In addition, fructose consumption in rats has prooxidant. Oral taurine supplementation prevents fructoseinduced. The blood pressurelowering effect of taurine appeared to be independent from its effect on glucose and lipid metabolism in this model.
Antihypertensive treatment with enrasentan sb217242 in. Restoration of a healthy intestinal microbiota normalizes. Histology of heart sections in different groups was examined by oil red o a and masson trichrome. Wistar rat dams were divided into control and fructose fed groups that received 10% fructose in their drinking water from 8 weeks of age and throughout pregnancy 10 weeks.
C no concentrations in the nts of fructose fed rats were determined throughout the 7day research. Rats with fructoseinduced hypertension were treated by oral gavage with either. Dietary vitamin e and c supplementation prevents fructose induced hypertension in rats article pdf available in molecular and cellular biochemistry 24112. Excess maternal salt or fructose intake programmes sex.
The mechanisms underlying fructoseinduced hypertension. Mitigation of insulin resistance by mangiferin in a rat model. Increased sugar consumption, especially fructose, is strongly related to the development of type 2 diabetes t2d and metabolic syndrome. Fructoseinduced cognitive dysfunction is associated with. Original article histopathology and urinary metabonomics. Second, in one study in overweight men, the rise in ambulatory blood pressure in response to 200 g of oral fructose per day for two weeks was blocked in those subjects concomitantly administered allopurinol. Frontiers high fructose intake during pregnancy in rats. Fructoseinduced hypertension in rats is concentration and duration. Maternal fructose drives placental uric acid production.
Hypertension developed was confirmed by systolic blood pressure sbp measured after 2 weeks of administration. To minimize insulin resistance due to fructose loading, we used lower concentrations of apple juice 2. Gut dysbiosis contribute to high fructose induced salt sensitive hypertension in spraguedawley rats. In addition to hypertension, fructosefed rats are also relatively insulin resistant, hyperinsulinemic, and hypertriglyceridemic. Cellular mechanisms of insulin resistance in rats with. All relevant data are within the paper and its supporting information files. Fructoseinduced hypertension in rats is concentration and durationdependent article pdf available in journal of pharmacological and toxicological methods 332. We recently showed that a high fructose diet containing vegetable oil but a normal sodiumpotassium ratio induced mild insulin resistance with decreased insulin receptor substrate1 tyrosine phosphorylation in the liver and muscle of normal rats. Saltsensitive dahl rats develop salt induced hypertension with decreased insulin sensitivity. Dawley rats are fed a high fructose diet, they develop hypertension that is accompanied by insulin resistance. Original article mangiferin diminishes chronic fructose. Fructose, insulin resistance, and metabolic dyslipidemia. Maternal fecal and blood samples were collected premating 9 weeks and during early gestational day 47 and late pregnancy gestational day 1921. Relative liver weight and plasma concentrations of glucose and triglycerides were also elevated after fructose.
The protective role of amla emblica officinalis gaertn. Rats fed with high fructose diet provide an animal model of insulin resistance and acquired form of systolic hypertension 4. Cinnamaldehyde and allopurinol reduce fructoseinduced. Maternal administration of probiotic or prebiotic prevents. After induction of diabetes, rats were divided into four groups for the oral ingestion of ni, nisln andor vehicles and their effects on blood glucose levels, oral glucose toler. High fructose intake may raise blood pressure webmd. High fructose diets have been documented to increase blood pressure in experimental rats 5. Bx for 4 weeks than those required for induction of type 2 diabetes as reported previously 24. Moley1 maternal metabolic diseases increase offspring risk for low birth weight and cardiometabolic diseases in adulthood. Hsieh ps, tai yh, loh ch, shih kc, cheng wt, chu ch 2005 functional interaction of at1 and at2 receptors in fructoseinduced insulin resistance and hypertension in rats.
Dietary vitamin e and c supplementation prevents fructose induced. Dietary lipoic acid supplementation prevents fructoseinduced hypertension in rats article in nutrition metabolism and cardiovascular diseases 106. The impact of fructose on renal function and blood pressure. Much of the popular and scientific media have already decided against fructose and calls for regulation and taxation come from many quarters. Nov 04, 2009 hypertension is the most common chronic disease in developed countries. Fxr mrna expression remained unaltered in all transplanted groups fig. B renal sna in fructose fed rats was measured on days 0, 3, and 7. Cardiovascular autonomic dysfunction and oxidative stress. Hypertension, a component of metabolicinsulin resistance, impaired glucose tolerance syndrome is an important.
Masoprocol lowers blood pressure in rats with fructose. Antihypertensive potential of coenzyme q10 via free. The present results showed that longterm fructose feeding. Cellular mechanisms of insulin resistance in rats with fructose. More recently, increased formation of reactive oxygen species and elevated levels of uric acid have been reported to contribute to fructoseinduced hypertension. Fructoseinduced insulin resistance and hypertension in rats. Excess maternal salt or fructose intake programmes sexspecific, stress and fructose sensitive hypertension in the offspring volume 115 issue 4 clint gray, sheila m. Rats given a diet high in fructose have elevated fasting insulin and glucose, elevated insulin response to oral glucose load, significantly elevated triglycerides and elevated blood pressure. Aug 02, 2008 the most salient feature of the present studies is the prevention of generation of fructose induced hypertension in mice lacking slc26a6 and in rats on a chloridefree diet figures 4 and 5, clearly establishing the role of dietary chloride and chlorideabsorbing transporter slc26a6 in the pathogenesis of fructose induced hypertension. Taurine prevents hypertension and increases exercise capacity. Fructose induced insulin resistance and hypertension in rats. A highfructose diet induces insulin resistance but not blood.
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